Article
Identification of mosquito avian-derived blood meals by polymerase chain reaction-heteroduplex analysis.
The American journal of tropical medicine and hygiene - 1 May 2002
Lee Joon Hak, Hassan Hassan, Hill Geoff, Cupp Eddie W, Higazi Tarig B, Mitchell Carl J, Godsey Marvin S, Unnasch Thomas R
Abstract excerpt
A polymerase chain reaction (PCR) heteroduplex assay (HDA) was developed to identify avian derived mosquito blood meals to the species level. The assay used primers amplifying a fragment of the cytochrome B gene from vertebrate but not invertebrate species. In Culex tarsalis fed on quail, PCR products derived from the quail cytochrome B gene were detected seven days post-engorgement. In an analysis of wild-caught...
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