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Article

Improved partitioning in aqueous two-phase system of tyrosine-tagged recombinant lactate dehydrogenase.

Protein expression and purification - 1 Jul 2002

Fexby Sara, Bülow Leif

Abstract excerpt

The partitioning of Bacillus stearothermophilus lactate dehydrogenase (LDH) in an aqueous two-phase system was studied. Particularly, the influence of tyrosine tags on the partitioning was evaluated. The hydrophobic effect, caused by the addition of tyrosine residues, was determined in a system based on dextran and the thermoseparating ethylene oxide-propylene oxide random copolymer (EO30PO70). Five different LDH...

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PMID: 12135559
DOI: 10.1016/s1046-5928(02)00008-6

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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