Article
Photobacterium damselae ssp. piscicida: detection by direct amplification of 16S rRNA gene sequences and genotypic variation as determined by amplified fragment length polymorphism (AFLP).
Diseases of aquatic organisms - 5 Apr 2002
Kvitt H, Ucko M, Colorni A, Batargias C, Zlotkin A, Knibb W
Abstract excerpt
A PCR protocol for the rapid diagnosis of fish 'pasteurellosis' based on 16S rRNA gene sequences was developed. The procedure combines low annealing temperature that detects low titers of Photobacterium damselae but also related species, and high annealing temperature for the specific identification of P. damselae directly from infected fish. The PCR protocol was validated on 19 piscine isolates of P. damselae...
Topics
- Animals
- Bass
- DNA, Bacterial
- Fish Diseases
- Gene Amplification
- Genetic Variation
- Genotype
- Gram-Negative Bacterial Infections
- Photobacterium
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- RNA, Ribosomal, 16S
- Sea Bream