Article
Positional cloning of rice semidwarfing gene, sd-1: rice "green revolution gene" encodes a mutant enzyme involved in gibberellin synthesis.
DNA research : an international journal for rapid publication of reports on genes and genomes - 28 Feb 2002
Monna Lisa, Kitazawa Noriyuki, Yoshino Rika, Suzuki Junko, Masuda Haruka, Maehara Yumiko, Tanji Masao, Sato Mizuho, Nasu Shinobu, Minobe Yuzo
Abstract excerpt
A rice semidwarfing gene, sd-1, known as the "green revolution gene," was isolated by positional cloning and revealed to encode gibberellin 20-oxidase, the key enzyme in the gibberellin biosynthesis pathway. Analysis of 3477 segregants using several PCR-based marker technologies, including cleaved amplified polymorphic sequence, derived-CAPS, and single nucleotide polymorphisms revealed 1 ORF in a 6-kb candidate...
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