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Assessment of nuclear and cytoplasmic maturation in in-vitro matured human oocytes

1 Apr 2002

Abstract excerpt

BACKGROUND: With improved prospects for the use of human oocyte in-vitro maturation in assisted reproductive technologies, the need to define more clearly the coordination of nuclear and cytoplasmic maturation has arisen. METHODS: Immunofluorescence and confocal microscopy were used to evaluate cell cycle-dependent modifications in chromatin and microtubules in human germinal vesicle oocytes (n = 455) undergoing...

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PMID: 11925398
DOI: 10.1093/humrep/17.4.1006

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