Article
Infrequent p16/CDKN2 alterations in squamous cell carcinoma of the oesophagus.
European journal of gastroenterology & hepatology - 1 Jan 2002
Giroux Marie-Agnès, Audrezet Marie-Pierre, Metges Jean-Philippe, Lozac'h Patrick, Volant Alain, Nousbaum Jean-Baptiste, Labat Jean-Paul, Gouérou Hervé, Ferec Claude, Robaszkiewicz Michel
Abstract excerpt
Loss of heterozygosity (LOH) on chromosome 9 and p16 (MTS1/CDKN2) gene mutations have been reported in various human cancers. The present study aimed to determine the prevalence of LOH in 100 oesophageal squamous cell carcinomas (OSCCs) by typing microsatellite loci and mutations of the p16 gene. The methods used included denaturing gradient gel electrophoresis (DGGE) and DNA sequencing of exon 2. LOH was found...
Topics
- Aged
- Carcinoma, Squamous Cell
- Chromosomes, Human, Pair 9
- Electrophoresis, Polyacrylamide Gel
- Esophageal Neoplasms
- Female
- Genes, p16
- Humans
- Loss of Heterozygosity
- Male
- Microsatellite Repeats
- Middle Aged
- Mutation