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Developmental potential of human spermatogenic cells co-cultured with Sertoli cells

1 Jan 2002

Abstract excerpt

BACKGROUND: Development of an in-vitro culture system capable of supporting human early germ cell differentiation would be important for treatment of azoospermic patients. METHODS: Sertoli cells, spermatogonia and spermatocytes were isolated from testicular biopsies of 61 non-obstructive azoospermic patients, and co-cultured using Vero cell conditioned medium only or supplemented with recombinant (r)FSH or rFSH...

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PMID: 11756382
DOI: 10.1093/humrep/17.1.161

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