Article
Genomic interspersions determine the size and complexity of transgene loci in transgenic plants produced by microprojectile bombardment.
Genome - 1 Aug 2001
Svitashev S K, Somers D A
Abstract excerpt
The structure of transgene loci in six transgenic allohexaploid oat (Avena sativa L.) lines produced using microprojectile bombardment was characterized using fluorescence in situ hybridization (FISH) on extended DNA fibers (fiber-FISH). The transgene loci in five lines were composed of multiple copies of delivered DNA interspersed with genomic DNA fragments ranging in size from ca. 3 kb to at least several...
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