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Article

Unusual Abundance of Atypical Strains Associated with Human Ocular Toxoplasmosis

1 Sept 2001

Abstract excerpt

To facilitate genotyping of Toxoplasma gondii in vitreous fluid of patients with severe or atypical ocular toxoplasmosis, polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) assays were developed for SAG3 (p43) and SAG4 (p18), 2 single-copy surface antigen genes. Together with strategies for SAG1, SAG2, and B1, multilocus RFLP analyses were performed on PCR-amplified parasite DNA...

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PMID: 11474426
DOI: 10.1086/322800

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