Article
Characterization of equine microsatellites and microsatellite-linked repetitive elements (eMLREs) by efficient cloning and genotyping methods.
DNA research : an international journal for rapid publication of reports on genes and genomes - 28 Feb 2001
Tozaki T, Mashima S, Hirota K, Miura N, Choi-Miura N H, Tomita M
Abstract excerpt
We performed efficient cloning and genotyping methods for isolation of a large number of polymorphic microsatellites. The methods contain the time-efficient cloning method of constructing microsatellite-enriched libraries and the economic genotyping method of fluorescent labeling of PCR products. Eighty novel equine microsatellites cloned were efficiently isolated from the enrichment library and analyzed for...
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