Human papillomavirus DNA detection in sperm using polymerase chain reaction

Abstract excerpt

OBJECTIVE: To detect human papillomavirus (HPV) in semen and find if sperm washing removes HPV DNA. METHODS: Amplification by nested polymerase chain reaction (PCR) was used to detect viral DNA sequences in semen samples from 85 volunteers. Forty-five men had historical or clinical evidence of genital HPV infection (study group) and 40 were healthy, clinically HPV-negative semen donors. RESULTS: We detected HPV...

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