Detection and identification of eight Trichinella genotypes by reverse line blot hybridization.

doi:10.1128/JCM.39.2.642-646.2001

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Article

Detection and identification of eight Trichinella genotypes by reverse line blot hybridization.

Journal of clinical microbiology - 1 Feb 2001

Rombout Y B, Bosch S, Van Der Giessen J W

Abstract excerpt

A reverse line blot (RLB) assay was developed to identify different Trichinella genotypes. The RLB assay accomplishes detection and specific identification of the different Trichinella genotypes and relies on hybridization of the amplified 5S ribosomal DNA intergenic spacer regions to specific, membrane-bound oligonucleotide probes. After one single amplification, we were able to detect and genetically identify...

Topics

Animals
DNA, Helminth
DNA, Ribosomal
Genotype
Humans
Introns
Nucleic Acid Hybridization
RNA, Helminth
RNA, Ribosomal, 5S
Trichinella
Trichinella spiralis
Trichinellosis

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Identifiers

PMID: 11158122
DOI: 10.1128/JCM.39.2.642-646.2001
PMCID: PMC87791

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