Article
Overexpression of auxin-binding protein enhances the sensitivity of guard cells to auxin.
Plant physiology - 1 Nov 2000
Bauly J M, Sealy I M, Macdonald H, Brearley J, Dröge S, Hillmer S, Robinson D G, Venis M A, Blatt M R, Lazarus C M, Napier R M
Abstract excerpt
To explore the role of auxin-binding protein (ABP1) in planta, a number of transgenic tobacco (Nicotiana tabacum) lines were generated. The wild-type KDEL endoplasmic reticulum targeting signal was mutated to HDEL, another common retention sequence in plants, and to KEQL or KDELGL to compromise its activity. The auxin-binding kinetics of these forms of ABP1 were found to be similar to those of ABP1 purified from...
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