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Article

A single PCR-restriction endonuclease analysis for rapid identification of Malassezia species

1 Nov 2000

Abstract excerpt

AIMS: The present study describes a system based on PCR and restriction endonuclease analysis (REA) to distinguish the seven currently recognized Malassezia species. METHODS AND RESULTS: Fifty-five representative yeast isolates were examined. A single primer pair was designed to amplify the large subunit ribosomal RNA (LSU rRNA) gene of the seven Malassezia species, and identification was achieved by digestion of...

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PMID: 11069645
DOI: 10.1046/j.1472-765x.2000.00839.x

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