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Article

PCR-based identification of MAT-1 and MAT-2 in the Gibberella fujikuroi species complex.

Applied and environmental microbiology - 1 Oct 2000

Steenkamp E T, Wingfield B D, Coutinho T A, Zeller K A, Wingfield M J, Marasas W F, Leslie J F

Abstract excerpt

All sexually fertile strains in the Gibberella fujikuroi species complex are heterothallic, with individual mating types conferred by the broadly conserved ascomycete idiomorphs MAT-1 and MAT-2. We sequenced both alleles from all eight mating populations, developed a multiplex PCR technique to distinguish these idiomorphs, and tested it with representative strains from all eight biological species and 22...

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PMID: 11010886
DOI: 10.1128/AEM.66.10.4378-4382.2000
PMCID: PMC92312

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