Article
PBF-2 is a novel single-stranded DNA binding factor implicated in PR-10a gene activation in potato.
The Plant cell - 1 Aug 2000
Desveaux D, Després C, Joyeux A, Subramaniam R, Brisson N
Abstract excerpt
Elicitor-induced activation of the potato pathogenesis-related gene PR-10a requires a 30-bp promoter sequence termed the ERE (elicitor response element) that is bound by the nuclear factor PBF-2 (PR-10a binding factor 2). In this study, PBF-2 has been purified to near homogeneity from elicited tubers through a combination of anion-exchange and DNA affinity chromatography. Evidence demonstrates that inactive PBF-2...
Topics
- Amino Acid Sequence
- Antibodies
- Base Sequence
- Binding Sites
- Cell Nucleus
- Chromatography, Affinity
- Cloning, Molecular
- DNA, Single-Stranded
- DNA-Binding Proteins
- Gene Expression Regulation, Plant
- Genes, Plant
- Genes, Reporter
- Models, Genetic