Article
Monitoring Piscirickettsia salmonis by denaturant gel electrophoresis and competitive PCR.
Diseases of aquatic organisms - 25 May 2000
Heath S, Pak S, Marshall S, Prager E M, Orrego C
Abstract excerpt
Reported strains of Piscirickettsia salmonis, a pathogen of salmonid fishes, were analyzed by amplifying part of the internal transcribed spacer (ITS) of the ribosomal RNA (rRNA) operon followed by denaturing gradient gel electrophoresis (DGGE) of the amplicons. All amplified fragments differing in sequence were distinguished by migration during DGGE. A simpler format, constant denaturant gel electrophoresis...
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