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Leukocyte detection in human semen using flow cytometry

1 Jun 2000

Abstract excerpt

This study set out to establish a new method, using flow cytometry, to evaluate leukocytes in semen. Ejaculates of 59 males, asymptomatic for genitourinary infections, were examined. Routine semen analyses were carried out as well as peroxidase and polymorphonuclear granulocyte-elastase detection. Leukocytes were detected combining flow cytometry and monoclonal antibodies (anti-CD45, anti-CD53). This technique...

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PMID: 10831564
DOI: 10.1093/humrep/15.6.1329

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