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Quantitative Analysis of Human Herpesvirus 8 Viral Load Using a Real-Time PCR Assay

1 Apr 2000

Abstract excerpt

We have developed a quantitative real-time PCR (TaqMan) assay aimed at measuring the cellular human herpesvirus 8 (HHV-8) DNA load in various clinical samples. Standard curves were obtained by serial dilutions of a control plasmid containing both HHV-8 (ORF73 gene) and the cellular target (human albumin gene). The assay appeared to be very sensitive (100% detection rate for at least 10 copies per well) and...

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PMID: 10747115
DOI: 10.1128/jcm.38.4.1404-1408.2000

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