Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Detection of Trypanosoma brucei gambiense in sleeping sickness suspects by PCR amplification of expression‐site‐associated genes 6 and 7

1 Oct 1999

Abstract excerpt

We have developed a sensitive and specific method to identify Trypanosoma brucei ssp. using PCR to amplify conserved expression-site-associated gene 6 and 7 DNA target sequences. Amplification of 10% of the DNA in a single trypanosome produced sufficient PCR product to be visible as a band in an agarose gel stained with ethidium bromide. We analysed 59 blood samples of serologically positive cases of sleeping...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Eosinophilic Disorders and Syndromes
Share to Research on Leishmaniasis Studies
Share to Trypanosoma species research and implications

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 10583899
DOI: 10.1046/j.1365-3156.1999.00465.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 8 closest

Detection of Trypanosoma brucei gambiense in sleeping sickness suspects by PCR amplification of expression‐site‐associated genes 6 and 7PMID 10583899Read ↗

Novel primer sequences for polymerase chain reaction-based detection of Trypanosoma brucei gambiense.Semantic proximity Read ↗

Molecular detection of trypanosomes in cattle in South America and genetic diversity of Trypanosoma evansi based on expression-site-associated gene 6.Semantic proximity Read ↗

Detection of Group 1 Trypanosoma brucei gambiense by Loop-Mediated Isothermal AmplificationSemantic proximity Read ↗

Identification of human-infective trypanosomes in animal reservoir of sleeping sickness in Uganda by means of serum-resistance-associated (SRA) geneSemantic proximity Read ↗

The serum resistance-associated gene as a diagnostic tool for the detection of Trypanosoma brucei rhodesiense.Semantic proximity Read ↗

Detection of trypanosome infections in the saliva of tsetse flies and buffy-coat samples from antigenaemic but aparasitaemic cattleSemantic proximity Read ↗

Comparative Sensitivity and Specificity of the 7SL sRNA Diagnostic Test for Animal TrypanosomiasisSemantic proximity Read ↗

Salivarian Trypanosomes Have Adopted Intricate Host-Pathogen Interaction Mechanisms That Ensure Survival in Plain Sight of the Adaptive Immune SystemSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.