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Article

Molecular epidemiological investigation using a randomly amplified polymorphic DNA assay of Burkholderia cepacia isolates from nosocomial outbreaks.

Journal of clinical microbiology - 1 Dec 1999

Okazaki M, Watanabe T, Morita K, Higurashi Y, Araki K, Shukuya N, Baba S, Watanabe N, Egami T, Furuya N, Kanamori M, Shimazaki S, Uchimura H

Abstract excerpt

We experienced two Burkholderia cepacia outbreaks over a 1-year period. During this period, 28 B. cepacia isolates were obtained from clinical specimens, and 2 were obtained from environmental specimens (i.e., from a nebulizer solution and a nebulizer tube). These 30 isolates were subjected to the PCR-based randomly amplified polymorphic DNA (RAPD) assay as well as to pulsed-field gel electrophoresis (PFGE). In...

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Identifiers

PMID: 10565889
DOI: 10.1128/JCM.37.12.3809-3814.1999
PMCID: PMC85819

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Source receipt: pubmed, retrieved 7 Sept 2026.

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