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Detection of Leishmania infantum in Dogs by PCR with Lymph Node Aspirates and Blood

1 Sept 1999

Abstract excerpt

The PCR technique was applied to the diagnosis of leishmaniasis in dogs, both serologically negative and positive. DNA was taken from lymph node aspirates and blood. The primers 13a and 13b, derived from Leishmania amazonies and Leishmania braziliensis kinetoplast DNA (kDNA), also amplified Leishmania infantum IPT1 constant region of minicircle kDNA. The amplified fragment is 116 bp long. It was cloned and the...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 10449478
DOI: 10.1128/jcm.37.9.2931-2935.1999

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