Article
In vitro development of Staphylococcus aureus biofilms using slime-producing variants and ATP-bioluminescence for automated bacterial quantification.
Luminescence : the journal of biological and chemical luminescence - 1 Jan 2000
Gracia E, Fernández A, Conchello P, Alabart J L, Pérez M, Amorena B
Abstract excerpt
In this work, a method was developed to establish Staphylococcus aureus biofilms on 96-well plates and automatically quantify viable cells within these biofilms by ATP-bioluminescence. Different strains were compared for biofilm formation. Cells from slime producing (SP) strain variants were more adherent (p < 0.001) and therefore more suitable for biofilm formation than non-slime producing original isolates. To...
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