Article
PCR-based genotyping of epidemic and preepidemic Trichoderma isolates associated with green mold of Agaricus bisporus.
Applied and environmental microbiology - 1 Jun 1999
Chen X, Romaine C P, Tan Q, Schlagnhaufer B, Ospina-Giraldo M D, Royse D J, Huff D R
Abstract excerpt
We used randomly amplified polymorphic DNA (RAPD)-PCR to estimate genetic variation among isolates of Trichoderma associated with green mold on the cultivated mushroom Agaricus bisporus. Of 83 isolates examined, 66 were sampled during the recent green mold epidemic, while the remaining 17 isolates were collected just prior to the epidemic and date back to the 1950s. Trichoderma harzianum biotype 4 was identified...
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