Article
Paradoxical stimulation of a DEG/ENaC channel by amiloride.
The Journal of biological chemistry - 28 May 1999
Adams C M, Snyder P M, Welsh M J
Abstract excerpt
Extracellular amiloride inhibits all known DEG/ENaC ion channels, including BNC1, a proton-activated human neuronal cation channel. Earlier studies showed that protons cause a conformational change that activates BNC1 and exposes residue 430 to the extracellular solution. Here we demonstrate that, in addition to blocking BNC1, amiloride also exposes residue 430. This result suggested that, like protons, amiloride...
Topics
- Acid Sensing Ion Channels
- Amiloride
- Animals
- Binding Sites
- Cysteine
- DNA, Complementary
- Degenerin Sodium Channels
- Epithelial Sodium Channels
- Humans
- Ion Channels
- Mesylates
- Microinjections
- Mutation
- Nerve Tissue Proteins
- Oocytes
- Protons
- Sodium Channels
- Xenopus laevis