Article
[Regulation of L-asparaginase biosynthesis in mutants of Bacillus mesentericus 43A, poorly growing in the presence of aspartic acid].
Voprosy meditsinskoi khimii - 1 Jan 2000
Eremenko V V, Mardashev S R, Tiul'panova E S, Evseev L P
Abstract excerpt
Biosynthesis of L-asparaginase (EC 3.5.1.1) was inhibited in the growing culture of Bac. mesentericus 43A on addition of L-aspartic acid (20 mM). My treatment with methyl nitrosourea (2 mg/ml) mutants were obtained, which grew poorly on aspartic acid used as the only source of carbon and nitrogen. The aspartic acid did not repress the asparaginase biosynthesis in 8 strains, found between the mutants. In six of...
Topics
- Asparaginase
- Aspartic Acid
- Bacillus
- Citrates
- Culture Media
- Culture Techniques
- Glutamates
- Mutation