Article
Two evolutionarily closely related ABC transporters mediate the uptake of choline for synthesis of the osmoprotectant glycine betaine in Bacillus subtilis.
Molecular microbiology - 1 Apr 1999
Kappes R M, Kempf B, Kneip S, Boch J, Gade J, Meier-Wagner J, Bremer E
Abstract excerpt
Biosynthesis of the compatible solute glycine betaine in Bacillus subtilis confers a considerable degree of osmotic tolerance and proceeds via a two-step oxidation process of choline, with glycine betaine aldehyde as the intermediate. We have exploited the sensitivity of B. subtilis strains defective in glycine betaine production against glycine betaine aldehyde to select for mutants resistant to this toxic...
Topics
- ATP-Binding Cassette Transporters
- Bacillus subtilis
- Bacterial Proteins
- Base Sequence
- Betaine
- Choline
- Evolution, Molecular
- Genotype
- Lipoproteins
- Models, Biological
- Models, Genetic
- Molecular Sequence Data
- Mutagenesis