Molecular typing of Candida spp. by random amplification of polymorphic DNA and analysis of restriction fragment length polymorphism of ribosomal DNA repeats.

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Article

Molecular typing of Candida spp. by random amplification of polymorphic DNA and analysis of restriction fragment length polymorphism of ribosomal DNA repeats.

The new microbiologica - 1 Jan 1999

Cresti S, Posteraro B, Sanguinetti M, Guglielmetti P, Rossolini G M, Morace G, Fadda G

Abstract excerpt

Random amplification of polymorphic DNA (RAPD) using an arbitrary oligonucleotide primer (5'-CGGTGCGACG) and analysis of restriction fragment length polymorphism of ribosomal DNA (rDNA-RFLP) after digestion of genomic DNA with restriction endonuclease EcoRI were investigated as tools for genotypic delineation beyond the species level of 91 Candida clinical isolates and four reference strains including 33 Candida...

Topics

Candida
DNA Fingerprinting
DNA, Fungal
DNA, Ribosomal
Genotype
Humans
Molecular Probe Techniques
Polymorphism, Restriction Fragment Length
Random Amplified Polymorphic DNA Technique

Source

Open in PubMed

Identifiers

PMID: 10190116

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