Evaluation of RT-PCR and reverse line blot hybridization for detection and genotyping F+ RNA coliphages from estuarine waters and molluscan shellfish.

doi:10.1111/j.1365-2672.2007.03646.x

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Article

Evaluation of RT-PCR and reverse line blot hybridization for detection and genotyping F+ RNA coliphages from estuarine waters and molluscan shellfish.

Journal of applied microbiology - 1 Apr 2008

Love D C, Vinjé J, Khalil S M, Murphy J, Lovelace G L, Sobsey M D

Abstract excerpt

AIMS: To evaluate a PCR-based detection and typing method for faecal indicator viruses (F+ RNA coliphages) in water and shellfish, and apply the method for better understanding of the ecology and microbial source tracking potential of these viruses. METHODS AND RESULTS: Water and shellfish samples were collected over 3 years at nine estuaries in the East, West and Gulf Coasts of the USA, providing 1033 F+ RNA...

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PMID: 18028362
DOI: 10.1111/j.1365-2672.2007.03646.x

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